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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Sample Type | RNA |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
GC-Rich PCR Performance | High |
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Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Invitrogen™ DynaMag™-PCR Magnet
The MAGnify™ ChIP system utilizes a novel magnet that is compatible with 0.2mL PCR strip tubes.
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Clean-up Reagent is used for enzymatic clean-up of amplified PCR product. It hydrolyses excess primers and nucleotides in a single step.
Shipping Condition | Dry Ice |
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Content And Storage | Shipped on dry ice. Store at -20°C. |
Protein Form | Recombinant |
Sample Type | PCR Products |
Research Category | Genetic Diseases |
For Use With (Application) | PCR Purification |
Product Line | ExoSAP-IT™ |
Thermo Scientific™ SureTect™ Brettanomyces spp. and Saccharomyces cerevisiae Multiplex PCR Assay
Rapidly detect and differentiate Brettanomyces spp. and S. cerevisiae in concentrate, semi-finished products, and environmental samples with the Thermo Scientific™ SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay.
Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications.
Content And Storage | -20°C |
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Applied Biosystems™ ExoSAP-IT™ Express PCR Product Clean-up Reagent
ExoSAP-IT™ Express PCR Product Clean-up Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. This enzymatic clean-up method offers precision, increased yield, and a faster turnaround time compared to alternative purification methods, such as spin columns or beads.
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Applied Biosystems™ HT ExoSAP-IT™ Fast High-Throughput PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications. Ideal for high-throughput PCR product cleanup using liquid handling platforms and multi-channel pipettes.
Content And Storage | Store at −20°C. Stable for one month at 4°C |
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Invitrogen™ DNAZap™ PCR DNA Degradation Solutions
DNAZap™ Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed.
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Size (Final Product) | Up to 5 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR) |
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
GC-Rich PCR Performance | Low |
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Format | Tube(s) |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Product Type | Exogenous Internal Positive Control Reagent |
Reaction Speed | Standard |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocin™ (ZeoR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Invitrogen™ Zero Blunt™ PCR Cloning Kit
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
Promoter | T7 |
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Content And Storage | Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt PCR |
Bacterial or Yeast Strain | TOP10 |
Vector | Blunt DNA Cloning Vectors |
Product Line | Zero Blunt™ |