Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)₁₆ included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.