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Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
2595.00 NOK - 7920.00 NOK
Specifications
| Detection Method | Gel Electrophoresis |
|---|---|
| Format | One-Step RT-PCR System |
| GC-Rich PCR Performance | High |
| PCR Method | 1-step RT-PCR |
| Polymerase | Platinum Taq DNA Polymerase |
| Code produit | Marque | No. of Reactions | Prix | Quantité et disponibilité | |||||
|---|---|---|---|---|---|---|---|---|---|
| Code produit | Marque | No. of Reactions | Prix | Quantité et disponibilité | |||||
|
10464022
|
Invitrogen™
12574018 |
25 Reactions |
2595.00 NOK
25 reactions |
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|
10082922
|
Invitrogen™
12574026 |
100 Reactions |
7920.00 NOK
100 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and Platinum Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 μg of total RNA.
Key advantages of SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
• Sensitive detection down to 0.01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
SuperScript III Reverse Transcriptase
SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is recombinant Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.
Note: This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.
Informations sur la commande
Shipping Condition: Dry ice
Spécification
| Gel Electrophoresis | |
| High | |
| Platinum Taq DNA Polymerase | |
| 1-Step RT-PCR | |
| SuperScript III | |
| Dry Ice | |
| Built-In Hot Start | |
| Reverse Transcription | |
| RNA |
| One-Step RT-PCR System | |
| 1-step RT-PCR | |
| 30 min. | |
| 50°C | |
| Reduced | |
| PCR Amplified cDNA | |
| Premixed Components | |
| Up to 4.5 kb |
For Research Use Only. Not for use in diagnostic procedures.
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