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One-Step Reverse Transcription PCR Reagents

One-Step Reverse Transcription PCR Reagents

An array of products designed to facilitate rapid, convenient reverse trancription PCR processes by simultaneously performing reverse transcription and amplification steps.
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1 – 8 of 8 results
1

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

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Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq DNA Polymerase
Format One-Step RT-PCR System
Size (Final Product) Up to 4.5 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
2

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
Concentration 4X
For Use With (Application) Pathogen Detection,Virus Detection,Real Time PCR (qPCR),Gene Expression
PCR Method 1-step RT-qPCR,Multiplex qPCR
Detection Method Primer-probe
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX™
Reaction Speed Fast or Standard
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath™
3

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
4

Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

5

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq High Fidelity
Format One-Step RT-PCR System
Size (Final Product) Up to 10 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
6

Invitrogen™ SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum SuperFi
Format Kit
Size (Final Product) Up to 14 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 45°C to 60°C
Technique 1-step RT-PCR
Starting Material RNA
7

Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR.

8

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Format Single-Tube
Form Liquid
Reaction Speed Fast or Standard
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
For Use With (Application) Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression
PCR Method 1-step RT-qPCR,Multiplex qPCR
Product Line TaqPath™
Detection Method Primer-probe