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Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

Brand: Invitrogen™ 10928042

Product Code. 10606343

9280.00 NOK / 100 reactions

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Description

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

Specifications

Content And Storage	• SuperScript II RT/Platinum Taq DNA Polymerase mix (100 μL) • 2X Reaction mix (3 x 1 mL) • 5 mM magnesium sulfate (500 μL) • 50 mM magnesium sulfate (1 mL) Store at –20°C.
Format	Kit
Polymerase	Platinum Taq DNA Polymerase
Technique	1-step RT-PCR
Quantity	100 rxns
Ribonuclease H Activity	Reduced
Final Product Type	PCR Amplified cDNA
No. of Reactions	100 Reactions
Reagent Type	Reverse Transcription
Starting Material	RNA

Detection Method	Gel Electrophoresis
PCR Method	1-step RT-PCR
Reaction Speed	30 to 50 min.
Optimal Reaction Temperature	55°C to 60°C
Reverse Transcriptase	SuperScript II
Shipping Condition	Wet Ice
Hot Start	Built-In Hot Start
Reaction Format	Master Mix
Size (Final Product)	Up to 7 kb

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Documents

Scientific Resources

Reference Materials

White Paper: Advanced analysis with the SuperScript IV One-Step RT-PCR System File size: 904.7 kB

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number2902375	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number10928042
Lot Number2491632	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number10928042
Lot Number2464609	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number10928042
Lot Number2538725	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number10928042
Lot Number2736737	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog Number10928042

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