
Thermo Scientificâ„¢ Pierceâ„¢ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Substrate | Horseradish Peroxidase |
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Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 1 to 2 hr. |
Product Line | Pierceâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate | Horseradish Peroxidase |
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Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 8 hr. |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ SuperSignalâ„¢ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate | Horseradish Peroxidase |
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Sensitivity | Mid-femtogram |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 24 hr. |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ Pierceâ„¢ Streptavidin Poly-HRP
Amplify signal and improve detection of biotinylated antibodies for IHC and other methods using this streptavidin conjugated with HRP polymers.

Thermo Scientificâ„¢ Pierceâ„¢ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
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Form | Powder |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
Product Line | Pierceâ„¢ |
Invitrogenâ„¢ XCell IIâ„¢ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes

Thermo Scientificâ„¢ Pierceâ„¢ High Sensitivity NeutrAvidinâ„¢-HRP
Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.

Specificity | Biotin |
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Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C. |
Target Molecule | Biotin |
Form | Liquid |
Enzyme | HRP (Horseradish Peroxidase) |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA |
Product Line | Pierceâ„¢ |
Detection Method | Colorimetric, Chemiluminescence, Chemifluorescence |
Invitrogenâ„¢ NuPAGEâ„¢ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

Thermo Scientificâ„¢ SuperSignalâ„¢ West Atto Ultimate Sensitivity Substrate
SuperSignalâ„¢ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Shipping Condition | Wet Ice |
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Substrate | Horseradish Peroxidase |
Sensitivity | Low-femtogram to high-attogram |
Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ Pierceâ„¢ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Sensitivity | Low picogram to mid-femtogram |
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Form | Liquid |
Product Type | Substrate |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierceâ„¢ |
Detection Method | Chemiluminescence, Fluorescence |
Thermo Scientificâ„¢ 1-Stepâ„¢ Ultra TMB-Blotting Solution
Thermo Scientificâ„¢ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
Thermo Scientificâ„¢ Pierceâ„¢ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Product Type | Transfer Buffer |
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Invitrogenâ„¢ Boltâ„¢ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Thermo Scientificâ„¢ 1-Stepâ„¢ NBT/BCIP Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.