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Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System
RNA UltraSense™ One-Step Quantitative RT-PCR System
Brand: Applied Biosystems™ 11732927
This item is not returnable.
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Description
The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/μl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:Applications:
Real-time quantitative amplification of RNA, including:
Specifications
The RNA UltraSense™ One-Step Quantitative RT-PCR System is supplied with the following components: 250 μl RNA UltraSense™ Enzyme Mix (includes SuperScript™ III RT, Platinum™ Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor); 2 x 1 ml RNA UltraSense™ 5X Reaction Mix; 300 μl 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml); 1 ml 50-mM magnesium sulfate (MgSO4 ); 100 μl ROX Reference Dye. Sufficient reagents are provided for 100 reactions. Store components at -20°C. Stability can be extended by storing at -80°C. ROX Reference Dye must be stored in the dark. | |
High | |
DNA Polymerase | |
Platinum™, SuperScript™, UltraSense™ | |
Dry Ice | |
100 Reactions |
Primer-probe | |
1-step RT-qPCR | |
Standard | |
One-Step Quantitative RT-PCR System | |
100 Reactions | |
RNA |
For Research Use Only. Not for use in diagnostic procedures.