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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Brand: Applied Biosystems™ A25742

2210.11 NOK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

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Product Code. 15350929

3315.00 NOK / 5mL

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Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

A dual hot-start mechanism for excellent specificity
Highly reproducible CTs over a broad dynamic range
Inclusion of UDG to help prevent carryover contamination
Stability of pre-assembled reactions for up to 72 hours
Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:

Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:

PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
In addition, this master mix provides efficient amplification over 6 logs of sample input
Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:

The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:

The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:

PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Specifications

Content And Storage	The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 x 5 mL tube, sufficient for 500 20-μL reactions (10 μL Master Mix per reaction). Store at 2–8°C in the dark.
Format	Tube
Polymerase	Dual-Lock Taq DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Type	Real Time PCR SYBR Master Mix
Shipping Condition	Wet Ice
For Use With (Application)	Gene Expression
No. of Reactions	500 Reactions
Volume	5 mL

Detection Method	SYBR
PCR Method	qPCR
Reaction Speed	Fast or Standard
Product Line	PowerUP™, SYBR™
Quantity	5 mL
Sufficient For	500 Reactions of 20 μL
Concentration	2X
Sample Type	DNA

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Documents

Product Information

Product Bulletins

Product Bulletin: PowerUp SYBR Green Master Mix File size: 379.3 kB

Product Bulletin: PowerUp SYBR Green Master Mix—exceptional specificity, broad instrument capability File size: 1.3 MB

Manuals

User Guide: PowerUp SYBR Green Master Mix - Universal 2X master mix for real-time PCR workflows Version: F.0 File size: 2.4 MB

Scientific Resources

Application Notes

White Paper: Design and optimization of SYBR Green assays File size: 282.7 kB

Brochures

Guidelines for comparing SYBR Green master mixes File size: 59.5 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2807100	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA25742,A25742_LITL18 PROMO
Lot Number2811271	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA25742,A25742_LITL18 PROMO
Lot Number2790262	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA25742,A25742_LITL18 PROMO
Lot Number2796576	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA25742,A25742_LITL18 PROMO
Lot Number2818831	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberA25742,A25742_LITL18 PROMO

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