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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual
Designed for cloning Taq-amplified PCR products directly from PCR reaction using overnight ligation step
Brand: Invitrogen™ KNM204040
This item is not returnable.
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Description
TA Cloning Kits use a pCR vector and yield ≥80% recombinants with ≥90% of the recombinants containing inserts.
- 3′-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- Choice of kanamycin or ampicillin selection
- Easy blue/white colony screening
- M13 forward and reverse primer sites for sequencing
- Manual not included
- Cloning Method: Restriction enzyme/MCS
Cloning, PCR Cloning
Specifications
| INVαF' | |
| Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual. | |
| One Shot TOP10 Chemically Competent E. coli |
| Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| 40 | |
| TA Cloning Vectors |