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DNA Cloning and Mapping and Mutagenesis

DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

2

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

3

Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit

Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

4

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

EDGE
5

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Promotions are available
EDGE
Shipping Condition Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART™
6

Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Promotions are available
EDGE
Product Type Rapid DNA Ligation Kit
Enzyme DNA Ligase
7

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Shipping Condition Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 15 fragments
Product Type Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART™
8

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15-60 minute isothermal reaction.

9

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

10

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

11

Thermo Scientific™ pTZ19R DNA

Perform DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system with this double-stranded closed circular high copy phagemid DNA.

12

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

13

Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit

The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

14

Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells

An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.

Content And Storage Dry Ice (-80°C)
Description Bacterial/Yeast Strain: DH10B
For Use With (Application) Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS)
15

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.