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Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

3945.00 NOK - 7400.00 NOK

Specifications

Product Type Chemically Competent Cells
Contains F' Episome Yes
Improves Plasmid Quality Yes (endA1)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10113902
View Documents Promotion Details
Invitrogen™
C303003
21 x 50 μL
3945.00 NOK
Each
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10503623
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Invitrogen™
C303006
42 x 50 μL
7400.00 NOK
Each
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Description

Description

One Shot TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are provided at a transformation efficiency of >1 x 109 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ Chemically Competent E. coli cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carry recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

One Shot TOP10F´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: IPTG induction is required for blue-white color screening.

Easy-to-use One Shot format
TOP10 F´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.

Genotype
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

Find the strains and formats that fit your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high throughput applications there are a variety of TOP10 strains in MultiShot formats.
• For ssDNA production try strains such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Chemically Competent Cells
Yes (endA1)
High Efficiency (>1 x 109 cfu/μg)
Not suitable for cloning unstable DNA
Low
Yes (recA1)
No
Trc, Tac, Lac
One Shot™
Yes
Yes (mcrA)
Yes (Streptamycin, Tetracycline)
Yes (lacZΔM15)
No
Dry Ice
E. coli (K12)
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For Research Use Only. Not for use in diagnostic procedures.