missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli
One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.
Brand: Invitrogen™ C303006
4233.24 NOK valid until 2024-12-31
Use promo code "21651" to get your promotional price.
This item is not returnable.
View return policy
Description
One Shot TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.
TOP10F´ competent cells are provided at a transformation efficiency of >1 x 109 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.
As a derivative of TOP10, TOP10F´ Chemically Competent E. coli cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carry recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
One Shot TOP10F´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 109 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Note: IPTG induction is required for blue-white color screening.
Easy-to-use One Shot format
TOP10 F´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.
Genotype
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG
Find the strains and formats that fit your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high throughput applications there are a variety of TOP10 strains in MultiShot formats.
• For ssDNA production try strains such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).
Order Info
Shipping Condition: Dry Ice
Specifications
| Chemically Competent Cells | |
| Yes (endA1) | |
| High Efficiency (>1 x 109 cfu/μg) | |
| Yes (Streptamycin, Tetracycline) | |
| Yes (lacZΔM15) | |
| No | |
| Dry Ice | |
| E. coli (K12) | |
| Tube | |
| 42 x 50 μL |
| Yes | |
| Yes (mcrA) | |
| • One Shot TOP10F' Chemically Competent E. coli (42 x 50 μL) Store Competent Cells at –80°C. • pUC19 plasmid (50 μL at 10 pg/μL) Store pUC19 plasmid at –20°C. • S.O.C. Medium (6 mL) Store S.O.C. Medium at 4°C or room temperature. |
|
| Not suitable for cloning unstable DNA | |
| Low | |
| Yes (recA1) | |
| No | |
| Trc, Tac, Lac | |
| One Shot™ |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction