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C32 (human amelanotic melanoma) Whole cell lysate, Denatured; Abnova

Brand:  Abnova L002V1.200ug

Product Code. 16099485

  • 1375.00 NOK / 200µg

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Description

Description

Western Blotting

Specifications

Specifications

Western Blot
Whole cell lysate (denatured)
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
200 μg
Human
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Skin
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
12.5% SDS-PAGE Stained with Coomassie Blue.
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
3 mg/mL
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Product Title
C32 (human amelanotic melanoma) Whole cell lysate, Denatured; Abnova > 50μg

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