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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Thermo Scientific™ Pierce™ FlexMix™ Calibration Solution is a mixture of 16 highly pure, ionizable components designed for both positive and negative ionization calibration using Orbitrap Tribrid Series 3.1 Instrument Control Software.
Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Protein Family | Avidin |
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Content And Storage | Upon receipt store product 4°C. Product is shipped at ambient temperature. |
Target Molecule | Biotin |
Form | Solid |
Label or Dye | Unlabeled |
Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays.
Product Line | Qubit™ |
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Detection Method | Fluorescence |
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
Assay | BCA Assay |
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Specificity | Not Target-Specific |
Content And Storage | Store at room temperature. |
Description | Pierce BCA Protein Assay Reagent A |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Obtain both positive and negative ionization calibration of Thermo Scientific™ Triple Stage Quadrupole instruments using the Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution, Extended Mass Range, which is a mixture of 14 highly pure, ionizable components (mass range: 69 m/z to 2800m/z).
Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Shipping Condition | Wet Ice |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Form | Powder |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 AĚŠ) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 AĚŠ |
PEGylated | No |
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Form | Solid |
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