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Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
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Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
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Content And Storage | Store desiccated at -20°C, protect from moisture |
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Description | Pierce NHS-Fluorescein |
Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
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Invitrogen™ ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (30X)
ReadyProbes Mouse-on-Mouse IgG Blocking Solution blocks endogenous binding when mouse IgG primary antibody is used to label mouse tissue.
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Thermo Scientific™ Pierce™ Biotin
Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).
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Thermo Scientific™ TMTpro™ 16plex Label Reagent Set
The Thermo Scientific TMTpro 16plex Label Reagent Set enables multiplexing of up to 16 samples for multiplexed protein identification and quantitation using tandem mass spectrometry (MS/MS).
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Workflow Step | Protein Labeling |
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Final Product Type | Peptide |
For Use With (Equipment) | Mass Spectrometer |
Label or Dye | Tandem Mass Tag™ (TMT) Labels |
Detection Method | Mass Spectrometry |
Product Line | TMT™ |
Thermo Scientific™ Pierce™ Streptavidin
Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.
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Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C. |
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Conjugate | Unconjugated |
Target Molecule | Biotin |
Product Line | Pierce™ |
Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Target Species | Human |
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Conjugate | Biotin |
Gene Symbol | GMCSF |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-250 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P10145 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3576 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-8, CXCL8, NAP-I |
Thermo Scientific™ Pierce™ Bradford Protein Assay Kit
Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Target Species | Human |
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Conjugate | Biotin |
Gene Symbol | DER4,Fit-1,Ly84,ST2L,St2,St2-rs1,T1,T1/ST2 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-200 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P05231 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme:Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3569 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-6 |
Thermo Scientific™ Micro BCA™ Protein Assay Kit
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
Assay | BCA Assay |
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Specificity | Not Target-Specific |
Content And Storage | Store at room temperature. |
Description | Pierce BCA Protein Assay Reagent A |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Thermo Scientific™ Pierce™ Bradford Plus Protein Assay Reagent
Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
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Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.