Isothermal Amplification Reagents
Isothermal Amplification Reagents
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Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Shipping Condition | Dry Ice |
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Polymerase | Lyo-ready Bst DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | ≤10 minutes |
Optimal Reaction Temperature | 65°C |
For Use With (Application) | LAMP,RT-LAMP |
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Shipping Condition | Dry Ice |
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Polymerase | Bst DNA Polymerase |
Includes Label or Dye | SYTO 9 Green Fluorescent Nucleic Acid Stain |
Reverse Transcriptase | SuperScript IV |
Product Type | Master Mix |
Reaction Time | 5 to 15 minutes |
Concentration | 2X |
Optimal Reaction Temperature | 65°C |
For Use With (Application) | LAMP,RT-LAMP |
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Shipping Condition | Dry Ice |
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Polymerase | phi29 DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | 4 hours |
Concentration | 10 U/μL |
Optimal Reaction Temperature | 30°C |
For Use With (Application) | MDA-WGA,RCA |
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Shipping Condition | Dry Ice |
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Polymerase | EquiPhi29 DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | 2 hours |
Concentration | 10 U/μL |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | MDA-WGA,RCA |
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Shipping Condition | Dry Ice |
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Form | Liquid |
Research Category | Nucleic Acid Amplification |
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Shipping Condition | Dry Ice |
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Polymerase | EquiPhi29 DNA Polymerase |
Format | Kit |
Form | Liquid |
Product Type | DNA Amplification Kit |
Reaction Time | Up to 2 hours |
Research Category | Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | RCA,MDA-WGA |
Invitrogen™ L.b. Cas12a Nuclease
L.b. Cas12a Nuclease for simple, quick and specific analysis of your samples.
Form | Liquid |
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Product Type | Stand-alone enzyme |
Research Category | Nucleic Acid Analysis |
Concentration | 10 μM |
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.