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LAMP Assays

LAMP Assays

Loop-mediated isothermal amplification (LAMP) is a methodology for nucleic acid amplification that can be used as a diagnostic tool for disease detection. This process can be carried out without the need for a thermal cycler and may, thus, be cost effective. Suitable for the detection of COVID-19.
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Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Includes LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
Detection Limit <250 copies/25 μL (Direct LAMP)
<100 copies/25 μL (Purified RNA-based LAMP)
Bead Type Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
For Use With (Equipment) Heating block
Enzyme SuperScript IV & proprietary Bst-derived DNA polymerase
Formulation Liquid. 5 components included in the kit.
Sample Type Saliva or Nasopharyngeal/Nasal Swab
Color During reaction color changes from purple to blue
Sample Volume Range of sample inputs
Key Functions Detects SARS-CoV-2 presence
For Use With (Application) SARS-CoV-2 surveilance
Detection Method Colorimetric readout