Real Time PCR Master Mixes

Real Time PCR Master Mixes
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Applied Biosystemsâ„¢ TaqManâ„¢ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Shipping Condition | Dry Ice |
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Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqManâ„¢ |
SYBRâ„¢ Fast SYBRâ„¢ Green Master Mix
Designed for speed, SYBRâ„¢ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

GC-Rich PCR Performance | High |
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Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOneâ„¢, Fast Mode, StepOnePlusâ„¢, Fast Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Label or Dye | SYBR Green |
Product Line | SYBRâ„¢ |
TaqManâ„¢ TaqManâ„¢ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystemsâ„¢ TaqManâ„¢ Gene Expression Master Mix.

Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Purity or Quality Grade | UP (Ultra Pure) |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR SystemStepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Universal PCR Master Mix (2X), no AmpEraseâ„¢ UNG
TaqManâ„¢ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 2 X |
Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq DNA Polymerase |
Format | Tube |
Product Type | Fast Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOneâ„¢, Fast Mode, StepOnePlusâ„¢, Fast Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | AmpEraseâ„¢, TaqManâ„¢ |
Applied Biosystemsâ„¢ Absolute Qâ„¢ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.

Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR, no UNG
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time.

Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade | UP (Ultra Pure) |
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Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOneâ„¢, Standard Mode |
Sample Type | Environmental Sample, Food |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal PCR Master Mix, no AmpEraseâ„¢ UNG
TaqManâ„¢ Universal PCR Master Mix, No AmpEraseâ„¢ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

Shipping Condition | Wet Ice |
---|---|
Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, GeneAmp 9600, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, GeneAmp 9600, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqPathâ„¢ ProAmpâ„¢ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Shipping Condition | Wet Ice |
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Polymerase | Taq DNA Polymerase |
Content And Storage | Store at 2°C to 8°C in the dark. |
Product Type | qPCR Master Mix |
For Use With (Equipment) | 7500 Fast System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, StepOneâ„¢ System |
Reaction Speed | Fast or Standard |
For Use With (Application) | Genotyping |
PCR Method | qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal Master Mix II, no UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

GC-Rich PCR Performance | High |
---|---|
Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Product Type | Universal Master Mix II |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Product Line | TaqManâ„¢ |
TaqManâ„¢ TaqManâ„¢ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.

GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Genotyping |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
Product Type | Genotyping Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 |
Sample Type | DNA (Genomic) |
Reaction Speed | Standard |
Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ SYBRâ„¢ Green Universal Master Mix
Everything needed for SYBRâ„¢ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | SYBRâ„¢ |
Detection Method | SYBR |
Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Detection Method | SYBR |
Applied Biosystemsâ„¢ PowerTrackâ„¢ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Shipping Condition | Dry Ice |
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Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, Bio-Rad CFX Connectâ„¢, Bio-Rad CFX384 Touchâ„¢, Bio-Rad CFX96 Touchâ„¢, BioRad CFX96â„¢, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiAâ„¢ 7 System, StepOneâ„¢ System, MJ Chromo4, MJ Opticon, StepOnePlusâ„¢ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | Applied Biosystemsâ„¢ |