Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 649 results

Gibco™ DPBS, calcium, magnesium

DPBS, calcium, magnesium

Pricing and Availability
Specifications

Regulatory Status	For in-vitro diagnostic use
Dilution	1 X
Content And Storage	2° to 8°C
Additive	No Sodium Pyruvate
Form	Liquid
Product Type	DPBS
pH	7 to 7.2
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture

Gibco™ DPBS, calcium, magnesium

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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Pricing and Availability
Specifications

Dilution	1 X
Additive	No Sodium Pyruvate
Form	Liquid
Product Type	DPBS
Classification	Animal Origin-Free
pH	7.0 to 7.2
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
For Use With (Application)	Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Solution Type	Dulbecco's Phosphate Buffered Saline
Product Line	Gibco™

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

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Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Dilution	10 X
Content And Storage	Store at room temperature.
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.4
Product Line	Ambion™

PBS, 10X Solution, Molecular Biology Grade, Thermo Scientific Chemicals

For biological research. Thermo Scientific™ PBS, 10X Solution, Molecular Biology Grade is 0.2μm filtered and dispensed into durable, square nalgene bottles and upright carton.

Pricing and Availability
Specifications

Form	Solution
Formulation	80.6 mM sodium phosphate, 19.4 mM potassium phosphate, 27 mM KCl and 1.37 M NaCl in high purity dH₂O
pH	7.4

Gibco™ PBS, pH 7.2

PBS, pH 7.2

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Pricing and Availability
Specifications

Dilution	1 X
Content And Storage	15°to 30°C
Additive	No Sodium Pyruvate
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.2
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 to 320 mOsm/kg

Gibco™ DPBS (10X), no calcium, no magnesium

DPBS (10X), no calcium, no magnesium

Pricing and Availability
Specifications

Dilution	10 X
Content And Storage	15°to 30°C
Additive	No Sodium Pyruvate
Form	Liquid
Product Type	DPBS
pH	6 to 7
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	2500 to 2900 mOsm/kg

Gibco™ PBS (10X), pH 7.4

PBS (10X), pH 7.4

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Pricing and Availability
Specifications

Regulatory Status	For in-vitro diagnostic use
Dilution	10 X
Content And Storage	15°to 30°C
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Capacity	500 mL
pH	7.3 to 7.5
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3100 mOsm/kg

Gibco™ PBS, pH 7.4 (flow cytometry grade)

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry and as a cell culture reagent.

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Pricing and Availability

Gibco™ HEPES, 1M Buffer Solution

HEPES, 1M Buffer Solution

Pricing and Availability
Specifications

Absorbance	UV light
Packaging	1 Each
Physical Form	Liquid
pH	7.2 to 7.5
Certificate of Analysis	FDA, ISO 13485 standards
Chemical Name or Material	HEPES buffer
Concentration	1 M
For Use With (Application)	Mammalian cell culture

Gibco™ DPBS, no calcium, no magnesium

DPBS, no calcium, no magnesium

Pricing and Availability
Specifications

Dilution	1 X
Content And Storage	15°to 30°C
Form	Liquid
Product Type	DPBS

Thermo Scientific Chemicals HEPES, 99%

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing and Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Gibco™ PBS (10X), pH 7.2

PBS (10X), pH 7.2

Pricing and Availability
Specifications

Regulatory Status	For in-vitro diagnostic use
Dilution	10 X
Content And Storage	15°to 30°C
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Capacity	500 mL
pH	7.1 to 7.3
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3200 mOsm/kg

Gibco™ HBSS (10X), no calcium, no magnesium, phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Pricing and Availability

Thermo Scientific Chemicals MES monohydrate, 98%

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Pricing and Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
MDL Number	MFCD00149409
SMILES	C1COCCN1CCS(=O)(=O)O.O
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Pricing and Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Life Science Buffers

Life Science Buffers

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Gibco™ DPBS, calcium, magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS, calcium, magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.2 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS (10X), no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ HEPES, 1M Buffer Solution Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS, no calcium, no magnesium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.2 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ HBSS (10X), no calcium, no magnesium, phenol red Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ DPBS, calcium, magnesium

Gibco™ DPBS, calcium, magnesium

Gibco™ PBS, pH 7.2

Gibco™ DPBS (10X), no calcium, no magnesium

Gibco™ PBS (10X), pH 7.4

Gibco™ HEPES, 1M Buffer Solution

Gibco™ DPBS, no calcium, no magnesium

Gibco™ PBS (10X), pH 7.2

Gibco™ HBSS (10X), no calcium, no magnesium, phenol red