Search results for "sepharose+iba"

IBA Lifesciences Strep-Tactin™ Sepharose™ Resin
Strep-Tactin™ Sepharose™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
dimMatrix | Strep-Tactin Sepharose |
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Cytiva Q Sepharose™ High Performance Chromatography Media
Designed for high intermediate and polishing steps in downstream processing
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 90μm |
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Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
Flow Rate | 150 to 600cm/hr. |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | Ni Sepharose excel chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion
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Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).
Promoter | CMV |
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Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
Product Type | Vector |
Constitutive or Inducible System | Constitutive |
Quantity | 5 μg |
For Use With (Application) | Episomal expression (oriP, EBNA-1); BM40 secretion signal |
Marker Gene | Ampicillin |
IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
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Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.
Promoter | Tet |
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Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
Product Type | Vector |
Quantity | 5 μg |
Inducing Agent | Inducible with anhydrotetracycline |
Concentration | 250 ng/μL |
Marker Gene | Ampicillin |
Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns
Designed for method optimization and parameter screening
Buffer | 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol |
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Height (Metric) | 100mm |
Content And Storage | 4° to 30°C |
Diameter (English) Inner | 0.3 in. |
Bed Volume | 4.7mL |
Column Type | Ion Exchange Column |
Diameter (Metric) Inner | 7.7mm |
Quantity | 1 x 4.7mL |
Material | Polypropylene |
For Use With (Application) | Optimization and parameter screening, ion exchange chromato graphy |
Max. Pressure | 1.5 bar |
Particle Size | 45 to 165μm |
Flow Rate | <450 cm/hr. |
Support Type | 6% Cross-linked Agarose |
Height (English) | 3.93 in. |
Cytiva Sepharose Agarose Gel Filtration Media
Choose from a wide range of bead-formed agarose-based gel filtration media. Cytiva Life Sciences™ Sepharose Agarose Gel Filtration Media shows good separation over a wide molecular weight range.
Format | Solid Bead |
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Product Type | Sepharose Agarose Gel Filtration Media |
Quantity | 1 L |
For Use With (Application) | Allow characterization or cleaning up of samples containing components of diverse molecular weight |
Cytiva Ni Sepharose™ High Performance Media
Precharged with Ni2+ for optimal purification of histidine-tagged proteins. Cytiva Lifescience™ Ni Sepharose™ High Performance Media shows high binding capacity and compatibility.
Particle Size | 34μm |
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Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose HP chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600 cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
Particle Size | 45 to 165 μm |
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pH Range | 4 to 13 |
Cytiva HiTrap™ CM Sepharose Fast Flow IEX Columns
Weak cation exchanger for small-scale protein purifications
Content And Storage | 4°C to 30°C in 20% Ethanol |
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Description | HiTrap CM FF |
For Use With (Application) | For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules |
Cytiva Phenyl Sepharose™ CL-4B Gel
For immunoglobulin purification and fractionation as well as immunoprecipitation procedures
Anhydrotetracycline, IBA Lifesciences
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