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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

Brand:  Thermo Scientific™ PV3176

Product Code. 12020176

  • 6460.00 NOK / 800 reactions
Estimated Shipment: 29-01-2025
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Description

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
Pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
800 x 20μL
Biochemical phospho-specific activity (no antibody)
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
384-well plate
Methylcoumarin
End Point
Dry Ice
Peptide-Based Substrate
Serine/Threonine Kinases
Z ́-LYTE™
All necessary reagents for performing primary or secondary screens
Serine/Threonine Kinase Assay
–80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
800 x 20μL, 800 x 20 μL assays
405/465, 535 nm
800 x 20 μL Reactions
BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8
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