QIAGEN QIAcard FTA PlantSaver

The 4 spot area QIAcard FTA PlantSaver format provides a laminated flap that allows you to vigorously crush plant or insect samples into the FTA matrix without damaging the FTA card.

Features:

• Simply pressing plant samples onto the FTA matrix or applying homogenate captures and stabilizes the DNA.
• Store and collect at room and high temperatures for convenient collection in the field.
• Smaller sample size: Only young leaves are required, reducing growing time and speeding research.
• Fast purification in 30 minutes: PCR-ready DNA can be purified on FTA in three simple steps, all in a single tube at room temperature.

Applications:

• Analyze plant DNA by PCR-based assays
• Detection of Genetically Modified (GM) organisms
• Marker selected breeding
• Varietal identification
• Phylogeny analysis
• Amplification of low copy number loci
• Invader™ assays
• Multiple displacement amplification
• Transgenic identification

Analysis:

• Plant tissue is physically crushed on the card and the DNA binds to the matrix.
• The chemical coating on the FTA card protects the DNA from degradation and allows the cards to be stored at room temperature for extended periods of time.
• To prepare the sample for PCR, the matrix is washed with two nontoxic aqueous buffers.
• The DNA remains bound to the matrix throughout purification and a small disk (1.2 or 2 mm in diameter) of the matrix provides enough template for PCR analysis.