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Gibco™ Viral Production Cells 2.0

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

Brand: Gibco™ A51218

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Product Code. 17661546

51100.00 NOK / Pack of 6

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Description

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
They are a core component of the AAV-MAX Helper-Free AAV Production System
The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Viral Production Medium and 10% DMSO
Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance

Features of the Viral Production Cells 2.0 include:

Clonal, 293F-derived, high-producer cell line
Absence of SV40 large T antigen
Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
Enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
Robust scalability and passage stability

Specifications

Cell State	Frozen
Content And Storage	6 cryovials, store in vapor phase liquid nitrogen
Cell Type	Kidney (Embryonic)
Form	Frozen
Format	6 vials
Media Recommendation	Gibco Viral Production Medium
Product Line	AAV-MAX
Quantity	1 mL
Source	Human

Concentration	1e7 cells/mL
Cell Line	Viral Production Cells 2.0
Culture Type	Suspension Cell Culture
Species	Human
For Use With (Application)	AAV Production
No. of Cells	1 x 10⁷ cells
Product Type	Viral Production Cells
Shipping Condition	dry ice for domestic shipment; liquid nitrogen for international shipment

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Gibco™ Viral Production Cells 2.0

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