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Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
TrypLE™ Select Enzyme (10X), no phenol red
Brand: Gibco™ A1217703
This item is not returnable.
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Description
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:
Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
Specifications
| EDTA | |
| Animal Origin-free | |
| Mammalian Cell Culture | |
| Cell Culture Dissociation Reagent | |
| Energy efficient, Sustainable packaging | |
| 270 - 320 mOsm/kg | |
| Gibco™ | |
| 20 x 100mL | |
| Room Temperature |
| Storage conditions: 15–30°C. Protect from light. Alternate storage conditions: 2–8°C or -5 to -20°C. Shipping conditions: Ambient. Shelf life: 24 months from date of manufacture. |
|
| 10 X | |
| Liquid | |
| No Phenol Red | |
| No Calcium, No Magnesium, No Sodium Phosphate | |
| 6.9 to 7.4 | |
| Cell Culture Grade | |
| Room Temperature Stable | |
| In vitro Bioassay |
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.