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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Brand:  Invitrogen™ 452640

1557.60 NOK valid until 2024-12-20
Use promo code "21651" to get your promotional price.


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Product Code. 15751927

  • 2600.00 NOK / Each

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Includes: Topoisomerase I-activated pCR™II-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water
This item is not returnable. View return policy

Description

Description

Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)
Kit Options: TOPO TA Cloning Dual Promoter Kits
TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:

  • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
  • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
  • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
  • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
  • Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
  • Provide your own cells (Cat. Nos. 450640 and 452640)

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Ampicillin (AmpR), Kanamycin (KanR)
Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Cloning and subsequent in vitro transcription
TOPO™
T7, SP6
TOPO-TA Vectors
TOPO™-TA
Kit
Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water
TA Cloning Kit
10 Reactions
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For Research Use Only. Not for use in diagnostic procedures.