missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Competent Cells
  5. Invitrogen™ TOP10 Electrocomp™ Kit
  6. 10226592

Invitrogen™ TOP10 Electrocomp™ Kit

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Brand:  Invitrogen™

 View more versions of this product

Product Code. 10226592

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products »

Explore more special offers
This item is not returnable. View return policy

Description

Description

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. 

The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

High-efficiency cloning and plasmid propagation
Contains enough electrocompetent cells for 75 x 40μL rxns or 150 x 20μL rxns
30 x 100 μL
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.