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  5. Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent
  6. 15265743

Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

Brand:  Thermo Scientific™ 89822


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Product Code. 15265743

  • 7050.00 NOK / 500mL

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Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
This item is not returnable. View return policy

Description

Description

  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Bacterial Protein Extraction Reagent
Store at 4°C.
500 mL
500 mL
B-PER™
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberAA403694 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number89822
Lot NumberZL405089 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number89822
Lot NumberSPECS Certificate TypeCertificate of Analysis Date27/12/2024 Catalog Number89822
Lot NumberZK403481 Certificate TypeCertificate of Analysis Date16/12/2024 Catalog Number89822
Lot NumberZK401610 Certificate TypeCertificate of Analysis Date16/12/2024 Catalog Number89822
Special Offers

Special Offers

  • Multiple Manufacturers

    Advance your proteomics research with essential tools
    Promo Code: 25977

    Expires:  2025-05-31

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Product Title
Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent > 500 mL

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