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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit

Express of functional proteins with much greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems using the Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit.

Brand: Thermo Scientific™ 88893

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Product Code. 15392607

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Description

The Thermo Scientific 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10μg protein/mL reaction.

High expression - up to 750μg/mL of expressed protein
Reproducible results - low variability between experiments
Fast - express high levels of protein with 6 hours to overnight incubation
Easier storage - allows you to store lyophilized CHO cell lysate at -20°C
Functional - obtain functionally active proteins, including those containing disulfide bonds
Easy-to-use - three reaction components together carry out transcription and translation in one step
Great for labeling - amenable to incorporation of labeled and unnatural amino acids
Vector compatibility - uses existing pT7CFE1 vectors for protein expression

The 1-Step CHO High-Yield IVT System uses extracts containing the robust translation machinery of the pharmaceutical industry standard Chinese Hamster Ovary (CHO) cell line to generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids, and energy-generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750μg/mL. The CHO IVT kit includes all of the components required for transcription and translation of a recombinant gene, including an optimized expression vector.
Includes:
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.
Requires:
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)
Applications:

Production of large amounts of recombinant protein for structural analysis
Generation of active proteins for downstream applications such as protein interaction studies
Rapid large-scale expression and characterization of mutant proteins
Production of viral particles
Incorporation of unnatural amino acids
Production of cytotoxic proteins

Specifications

Formulation	Multi-component kit
Yield	750 Times greater yield per mL
Sufficient For	2 Reactions of 100μL each
Content And Storage	Store in ultra-cold freezer (-68°C to -85°C)

Kit Contents	1-Step™ CHO High-Yield IVT Kit, 2 x 100μL, Cell-Free Expression, Sufficient for 2 Reactions Of 100μL Each, –68 to –85°C Store In Ultra-Cold Freezer, Production of Large Amounts of Recombinant Protein for Structural Analysis
Type	1-step CHO High-Yield IVT Kit
Quantity	2 x 100 μL
For Use With (Application)	Protein biology, protein expression, cell-free (In-vitro) expression, In-vitro translation, production of large amounts of recombinant protein for structural analysis, generation of active proteins for downstream applications such as protein interaction s

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Documents

Product Information

Manuals

User Guide: 1-Step CHO High-Yield IVT Trial Kit Version: 2595 (7 Jan 2015) File size: 438.1 kB

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