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Thermo Scientific™ Tango™ OPRM1-bla U2OS DA Assay Kit
The Tango™ OPRM1-bla U2OS DA (Division Arrested) cells contain the human Opioid Receptor Mu 1 (OPRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Brand: Thermo Scientific™ K1599
This item is not returnable.
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Description
The Tango™ OPRM1-bla U2OS DA (Division Arrested) cells contain the human Opioid Receptor Mu 1 (OPRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ OPRM1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of DAMGO. In addition, Tango™ OPRM1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_000914 | |
| Division-Arrested Cells | |
| Tail from AVPR2 GPCR | |
| Fluorescence | |
| OPRM1 | |
| Single Cell Clone Origin | |
| 1 Plate | |
| U2OS | |
| Division-Arrested Cells Assay Kit | |
| Tango™ |
| Cell-based beta-arrestin recruitment | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ OPRM1-bla U2OS DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • Tango™ OPRM1-bla U2OS Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The Tango™ OPRM1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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| Tango™ OPRM1-bla U2OS DA Assay Kit, 1 Plate, ∽8,000,000 cells/vial Quantity of Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: Tango™ OPRM1-bla U2OS DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, Tango™ OPRM1-bla U2OS Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis | |
| Tango™ | |
| BLA (Beta-Lactamase) | |
| OPRM1 | |
| U2OS | |
| GPCR |
For Research Use Only. Not for use in diagnostic procedures.