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Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Brand: Thermo Scientific™ A38554
This item is not returnable.
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Description
- Sensitivity: low-femtogram to high-attogram
- Stability: eight hours for working solution, one year for kit at 4°C
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: up to four hours
- Recommended primary antibody concentration: 1:1000 to 1:5000 dilution (0.2 to 1.0 μg/mL)
- Recommended secondary antibody concentration: 1:100000 to 1:250000 dilution (4 to 10 ng/mL)
Specifications
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 20 mL | |
| Low-femtogram to high-attogram | |
| • Substrate Solution, 10 mL • Stable Peroxide Solution, 10 mL Sufficient for two mini-gel blots; 200 cm2 of membrane. Store reagents at 2–8°C. |
| Chemiluminescence | |
| Western Blot | |
| SuperSignal™ | |
| Horseradish Peroxidase | |
| 2 Mini-Gel Blots, 200 sq. cm of Membrane | |
| Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.