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IBA Lifesciences Strep-Tactin™ Magnetic Microbeads
IBA Lifesciences GmbH Strep-Tactin™ Magnetic Microbeads enable a rapid and economic cell isolation.
Brand: IBA Lifesciences 6-5510-050
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Beschreibung
- Strep-Tactin™ Magnetic Microbeads are microspheres covalently linked to Strep-Tactin™.
- They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer™) or surface marker-specific cells (Fab-TACS™/Nano-TACS™).
- To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag™ (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin™.
- Strep-Tactin™ multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells.
- The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker.
- The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface.
- The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.
Spezifikation
| 750 μL | |
| Suspension in Dulbeccos phosphate buffered saline (DPBS) without Ca and Mg, contains KCl and 0.1% sodium azide | |
| 1 to 3 μm | |
| Magnetic Affinity Bead |
| Strep-Tactin™ covalently linked to polysaccharide coated magnetic microbeads | |
| 2°C to 8°C, do not freeze | |
| 750 μL for isolation out of 5 x 108 cells |
For Research Use Only.