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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination.

Specifications

Format	Liquid
For Use With (Equipment)	StarGate™ System, Insect Host Cells
Inducing Agent	BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
pH	8
Promoter	Polyhedrin

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Products 27

Product Code	Brand	Description	Protein Tag	Includes	Price	Quantity & Availability
Product Code	Brand	Description	Protein Tag	Includes	Price	Quantity & Availability
15623828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4800-001	pLSG-IBA wt1	Untagged	pUC ori, Ampicillin resistance	N/A	N/A

15633828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4801-001	pLSG-IBA wt2	Untagged	BM40 signal, pUC ori, Ampicillin resistance	N/A	N/A

15643828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4803-001	pLSG-IBA 3	Strep-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15653828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4805-001	pLSG-IBA 5	Strep-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15663828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4823-001	pLSG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15673828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4825-001	pLSG-IBA 25	GST Tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15683828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4833-001	pLSG-IBA 33	His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15693828 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4835-001	pLSG-IBA 35	His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15603838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4843-001	pLSG-IBA 43	Strep-tag for C-terminal and His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15613838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4845-001	pLSG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15623838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4862-001	pLSG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15633838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4863-001	pLSG-IBA 63	FLAG-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15643838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4864-001	pLSG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15653838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4865-001	pLSG-IBA 65	FLAG-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15663838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4902-001	pLSG-IBA 102	Twin-Strep-tag for C-terminal	BM40 signal, pUC ori, Ampicillin resistance	N/A	N/A

15673838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4903-001	pLSG-IBA 103	Twin-Strep-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15683838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4904-001	pLSG-IBA 104	Twin-Strep-tag for N-terminal	BM40 signal, pUC ori, Ampicillin resistance	N/A	N/A

15693838 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4905-001	pLSG-IBA 105	Twin-Strep-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15603848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4923-001	pLSG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15613848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4942-001	pLSG-IBA 142	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal	BM40 signal, pUC ori, Ampicillin resistance	N/A	N/A

15623848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4943-001	pLSG-IBA 143	Twin-Strep-tag for C-terminal and His Tag (6x) for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15633848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4944-001	pLSG-IBA 144	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	BM40 signal, pUC ori, Ampicillin resistance	N/A	N/A

15643848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4945-001	pLSG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15653848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4962-001	pLSG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15663848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4964-001	pLSG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15673848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4967-001	pLSG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal	pUC ori, Ampicillin resistance	N/A	N/A

15683848 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4968-001	pLSG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal	pUC ori, Ampicillin resistance	N/A	N/A

Description

pLSG-IBA Baculo virus (insect cells) expression vectors

The pLSG-IBAwt1 vector carries the Polyhedrin promoter for high-level expression in insect cells.

Co-transfection with BacPAK6 linearized AcMNPV DNA (Clontech) or circular flashBAC modified AcMNPV DNA (Oxford Expression Technologies) allows the generation of recombinant baculovirus at very high efficiency through reconstitution of an essential gene (ORF 1629) and elimination of wild type virus to great extent.
Cloning and expression of recombinant proteins in insect cells
Polyhedrin promoter drives high-level expression of the insert
Ampicillin resistance and ColE1 origin of replication (pUC) support propagation in E. coli

Please note that cloning into pLSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pLSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Format	Liquid
Inducing Agent	BacPAK6 linearized A cmNPV DNA (Clontech) or circular flashBAC modified A cmNPV DNA (Oxford Expression Technologies)
Promoter	Polyhedrin
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Concentration	250 ng/μL
Color	Clear

For Use With (Equipment)	StarGate™ System, Insect Host Cells
pH	8
Product Type	Acceptor Vector for bacula (insect cell) expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Quantity	5 μg
For Use With (Application)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

Safety and Handling

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IBA Lifesciences StarGate pLSG-IBA Vectors for Baculo

Specifications

Description

Specifications

Safety and Handling

Documents