Novex™ SelectFX™ Alexa Fluor™ 488 Endoplasmic Reticulum Labeling Kit, for fixed cells

Description

The SelectFX Alexa Fluor 488 Endoplasmic Reticulum Labeling Kit provides all the reagents needed to label the endoplasmic reticulum in fixed cells. To detect the ER, the kit uses a primary antibody directed against the ER-associated protein disulfide isomerase (PDI) and an Alexa Fluor 488 dye-labeled secondary antibody. The fluorescence can be observed using the same optical filters used for fluorescein. The kit also includes cell fixative and permeabilization reagents and protocols for mammalian cell preparation and staining.

Kit Attributes:

•Applications: Validated application for SelectFX Alexa Fluor 488 Endoplasmic Reticulum Labeling Kit is endoplasmic reticulum staining.
•Format: Slide(s)
•Detection Method: Fluorescence microscopy
•Product Size: One kit

The network of folded membranes that comprises the endoplasmic reticulum (ER) in all eukaryotic cells constitutes greater than half of the total membrane in the average animal cell. The branching tubules and flattened sacs of the ER come in two forms: membrane bound by ribosomes (rough ER) or unbound by ribosomes (smooth ER). The ER has a central role in lipid and protein synthesis, protein chaperoning and folding, and calcium homeostasis. Abnormal protein folding in the ER is implicated in a number of diseases including cystic fibrosis and Alzheimer's disease.

The SelectFX Alexa Fluor 488 Endoplasmic Reticulum Labeling Kit provides all the reagents needed to label the ER in fixed cells. To detect the ER, the kit uses a primary antibody directed against the ER-associated protein disulfide isomerase (PDI) and an Alexa Fluor 488 dye-labeled secondary antibody; fluorescence is observed using standard fluorescein filters. The kit also includes cell fixative and permeabilization reagents and protocols for mammalian cell preparation and staining.