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Cytiva rProtein A Sepharose™ Fast Flow Affinity Media
Designed for purification of monoclonal and polyclonal antibodies at labs and process scales
Brand: Cytiva 17-1279-02
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Description
- Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
- High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
- No mammalian components involved during manufacturing process
- Matrix: 4% cross-linked agarose
- Average particle size: 90μm (d50V)
- Ligand: 6mg rProtein A/mL medium (E. coli)
- Binding capacity: ∽50mg human IgG/mL media
- pH stability working range: 3 to 10
- Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed
Specifications
25 mL | |
For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales | |
50 mg Human lgG/mL | |
150 to 250 cm/Hoμr | |
9.84 in. | |
100 kPa | |
2 to 9 | |
Recombinant Protein A (E. coli) |
Stable in aqueous Buffers commonly used in Protein A Chromatography | |
2°C to 8°C | |
rProtein A Sepharose Fast Flow Media | |
60 to 165 μm | |
25 cm | |
4% Cross-Linked Agarose | |
20% Ethanol |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- rProtein A Sepharose
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
- UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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