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Gibco™ RPMI 1640 Medium, HEPES, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Brand: Gibco™ 42401042
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Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
With: Phenol Red, HEPES
Without: L-glutamine
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
| ISO 13485 standards, FDA | |
| Roswell Park Memorial Institute (RPMI) 1640 Medium, 10 x 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Phenol Red, HEPES, 12 Months Shelf Life, 270 to 310mOsm/kg Osmolality, For Mammalian Cell Culture | |
| Liquid | |
| Phenol Red, HEPES | |
| Mammalian cell culture | |
| 7.0 to 7.4 |
| 2 to 8°C | |
| 1 X | |
| Roswell Park Memorial Institute (RPMI) 1640 Medium | |
| No Glutamine, No Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 500 mL |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.