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Thermo Scientific™ RPMI 1640 Medium for SILAC

Product Code. 15899041 Shop All Thermo Scientific Products
6 x 500 mL, Pack of 6
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Quantity:
6 x 500 mL
Unit Size:
Pack of 6
This item is not returnable. View return policy
This item is not returnable. View return policy

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Features of RPMI Media for SILAC:
  •  Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—media are sterile, endotoxin-free and cell culture-compatible
TRUSTED_SUSTAINABILITY

Specifications

Material Cell Lysate
Product Type Media
Content And Storage Store at 2–8°C.
Label or Dye Mass Isotope of Amino Acid
Shipping Condition Room Temperature
Starting Material Cell Lysate
Final Product Type Liquid Medium
Quantity 6 x 500 mL
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.

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