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Invitrogen™ Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

3395.00 NOK - 8560.00 NOK

Specifications

Detection Method Fluorescence
Excitation/Emission 500/525
For Use With (Equipment) Microplate Reader
Shipping Condition Room Temperature
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Products 3
Product Code Brand Product Type Quantitation Range Price Quantity & Availability  
Product Code Brand Product Type Quantitation Range Price Quantity & Availability  
10207502
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Invitrogen™
R11490
Quant-iT RiboGreen RNA Kit 1 to 200 ng
8560.00 NOK
1mL
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15797239
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Invitrogen™
R11491
Quant-iT RiboGreen RNA Reagent 1 to 200 ng
7310.00 NOK
1mL
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10694633
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Invitrogen™
R32700
RediPlate 96 RiboGreen RNA Kit 3 to 200 ng
3395.00 NOK
1 set
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.

Workflow 

This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection.  To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL.  RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.

Applications

Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.

Specifications

Specifications

Fluorescence
500/525
Microplate Reader
Room Temperature
Videos
SDS
Documents

Documents

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