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Invitrogen™ RAGE (MOK) Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHMOKX5
This item is not returnable.
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Description
The Human RAGE (Hu MOK) ELISA quantitates Hu MOK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MOK. Principle of the method The Human MOK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
Specifications
| Q9UQ07 | |
| 3 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 5891 | |
| <12% | |
| Assay Diluent Concentrate, SAV-HRP, Standard, Adhesive Plate Covers, Pre-coated 96 well plates, Stop Solution, Biotinylated Detection Antibody, Wash Buffer, Chromogen | |
| 5 x 96 Tests | |
| Signal Transduction | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 2.06 pg/mL to 1500 pg/mL | |
| 3 pg/mL to 1500 pg/mL | |
| Biotin | |
| Kinase & Inhibitors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Colorimetric Microplate Reader | |
| MOK | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
| RAGE, RAGE-1, RAGE1, STK30 | |
| 1 hr. 20 min. |
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