Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
Brand: Invitrogen™ 12361250
26487.99 NOK valid until 2025-06-21
Use promo code "24112" to get your promotional price.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules.
Features of Platinum SuperFi II DNA Polymerase include:
Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.
Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.
Applications
| Five boxes of Platinum SuperFi II Green PCR Master Mix, each containing: • Platinum SuperFi II DNA Polymerase (500 μL) • 5X SuperFi II Buffer (6 x 1.25 mL) Store at –20°C. |
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| Platinum SuperFi II DNA Polymerase | |
| 5 x 500 reactions | |
| Hot-start PCR, High-fidelity PCR | |
| Built-In Hot Start | |
| Blunt | |
| 20 kb or less |
| High | |
| Fast | |
| Dry Ice | |
| >300X | |
| 5 x 500 Reactions | |
| Standalone |
Expires: 2025-07-04
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