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pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Brand: Invitrogen A15005

Product Code. 13406426

2265.00 NOK / 100µg

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Description

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

Transfection efficiency

Cell confluency

Antibiotic selection conditions

Quality and concentration of DNA

Retargeting vector to integrase vector ratio

We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.

Specifications

Constitutive or Inducible System	Inducible
Inducing Agent	Tetracycline
Product Type	Mammalian Expression Vector
Selection Agent (Eukaryotic)	Geneticin™ (G-418)
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Quantity	100 μg
Product Line	Jump-In™

Delivery Type	Transfection
Promoter	CMV/TO
Reporter Gene	GFP (EmGFP)
Content And Storage	pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag	GFP (EmGFP)
Vector	Jump-In Vectors
For Use With (Application)	Stable Cell Line Development, Targeted Integration, Regulated Expression

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pJTI™ R4 EXP CMV-TO EmGFP pA Vector

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