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Thermo Scientific™ TRITC (5/6-tetramethyl-rhodamine isothiocyanate), mixed isomer Product Code.: 11809370
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Thermo Scientific™ TRITC (5/6-tetramethyl-rhodamine isothiocyanate), mixed isomer

Product Code. 11809370 Shop All Thermo Scientific Products
10 mg, 10mg
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Quantity:
10 mg
Unit Size:
10mg
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Product Code. 11809370

Brand: Thermo Scientific™ 46112

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Description

Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.

Thermo Scientific™ TRITC and Rhodamine Labeling Reagents and Kits are high-performance derivatives of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Rhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery

Includes:

NHS-Rhodamine Antibody Labeling Kit (Mfr. No. 53031)

NHS-Rhodamine, 3 vials; Borate Buffer, 1mL; Purification Resin, 5mL; Spin Columns, 6 each; Collection Tubes, 12 each

TRUSTED_SUSTAINABILITY

Specifications

Description Pierce TRITC
Quantity 10 mg
Product Type TRITC
Solubility DMF (Dimethylformamide)
Content And Storage Storage: Upon receipt store at -20°C. Product is shipped at ambient temperature. Store fluorescent dye in foil pouch with desiccant to protect from light and moisture.
Chemical Reactivity Amine
Reactive Moiety Isothiocyanate
Label or Dye Rhodamine Isomers, TMR (Tetramethylrhodamine), TRITC Isomers
Label Type Classic Dyes
Product Line Pierce™
Emission 575
Excitation 552
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