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Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Brand: Thermo Scientific™ 23460
This item is not returnable.
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Description
Thermo Scientific™ Pierce Sulfhydryl Addition Kit is a complete set of reagents, including SATA, to enable modification of primary amines to free sulfhydryl groups for use in heterobifunctional crosslinking techniques.
Highlights:
- Reacts with primary amines to add protected sulfhydryls
- An easy deprotection step generates a free sulfhydryl group
- Thermo Scientific™ Sulfhydryl Addition Kit contains all necessary components for thiolation, deprotection, purification and quantitation
Specifications
| Amine | |
| Sulfhydryl Group | |
| Pierce™ | |
| Active Ester, Succinimidyl Ester, NHS Ester | |
| Powder | |
| 1 Reaction | |
| Crosslinker |
| Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein • SATA, 2 mg • Hydroxylamine·HCl, 5 mg • Conjugation Buffer Stock (10X), 20 mL • PBS pack (makes 500 mL), 1 pack • Dimethylformamide (DMF), 1 mL • Dextran Desalting Column, 1 x 5 mL • Column Extender, 1 • Ellman's Reagent (DTNB), 2 mg • Cysteine·HCl, 20 mg Store at 4°C. |
|
| Conjugating Groups | |
| Heterobifunctional | |
| Short (<10 Å) | |
| SATA | |
| DMF, DMSO |
For Research Use Only. Not for use in diagnostic procedures.