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Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads Product Code.: 10150874

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Product Code. 10150874 Shop All Thermo Scientific Products
1 mL, Each
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Quantity:
1 mL
5 mL
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Each
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Product Code. 10150874

Brand: Thermo Scientific™ 88816

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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules.

Features of Streptavidin Magnetic Beads:

  • High-performance beads—non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike
  • Stable immobilization chemistry—streptavidin is immobilized using leach-resistant chemistry
  • High capacity—superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples
  • Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses
  • Superior performance—nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment

Applications:

  • Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources
  • Co-immunoprecipitate interaction complexes using biotinylated antibodies
  • Capture protein-protein interactions in pull-down assays using biotinylated 'bait' proteins
  • Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts
  • Capture single-stranded biotinylated DNA oligos
  • Isolate biotinylated PCR products

These streptavidin magnetic beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand. The iron oxide, super-paramagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads.

Pierce Streptavidin Magnetic Beads use a recombinant form of streptavidin with a mass of 53kDa and a near-neutral isoelectric point (pI). The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptavidin has no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine·HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 1 mL
Concentration Slurry: 10 mg/mL, 1% solids
Content And Storage Upon receipt store at 4°C. Product is shipped on an ice pack.
Particle Size 1 ÎĽm
Form Liquid
Product Line Pierce™
Quantity 1 mL
Protein Form Recombinant
Target Biotin
Type Magnetic Affinity Bead
Ligand Type Streptavidin
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZJ401549 Certificate TypeCertificate of Analysis Date31/10/2024 Product Code88816
Lot NumberZI394055 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code88816
Lot NumberZH390905 Certificate TypeCertificate of Analysis Date12/09/2024 Product Code88816
Lot NumberZG387466 Certificate TypeCertificate of Analysis Date08/08/2024 Product Code88816
Lot NumberZG387023 Certificate TypeCertificate of Analysis Date08/08/2024 Product Code88816

For Research Use Only. Not for use in diagnostic procedures.

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