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Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit Product Code.: 12182240
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Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Product Code. 12182240 Shop All Thermo Scientific Products
1000 Reaction Kit, Each
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Quantity:
100 Reaction Kit
1000 Reaction Kit
Unit Size:
Each
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Product Code. 12182240

Brand: Thermo Scientific™ 16167

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Description

Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.

Thermo Scientific™ Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of Renilla luciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.

Highlights:

  • Sensitive – highly sensitive detection of Green Renilla luciferase activity

  • Stable – increased signal stability compared to flash assays

  • Simple – does not require a luminometer with injectors

  • Compatible – assay reagents compatible with other Renilla luciferases

  • Automation-friendly – amenable to high throughput assays

  • Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

  • Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan™ Ascent and Varioskan™ Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

TRUSTED_SUSTAINABILITY

Specifications

Type Assay Kit
Target Luciferase, Green Renilla Luciferase
Quantity 1000 Reaction Kit
Content And Storage Upon receipt store kit at -80°C or store individual components as indicated above.
• Renilla Glow Assay Buffer, 50 mL, store at 4°C
• Coelenterazine (100X), 0.5 mL, store at -80°C
• 2X Cell Lysis Buffer, 60 mL, store at room temperature
Assay Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Detection Method Bioluminescence
For Use With (Equipment) Luminometer (Microplate)
Format 384-well plate, 96-well plate
Label Type Enzyme Labeled
Substrate Properties Chemical Substrate
Technique Enhanced Chemiluminescence
Substrate Type Luciferase Substrate
Product Line Pierce™
Substrate Coelenterazine
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Safety and Handling

compliance-icons compliance-icons compliance-icons
Product Identifier
  • Renilla Luciferase Glow Assay Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.
 Precautionary Statement
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Methanol

For Research Use Only. Not for use in diagnostic procedures.

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