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Thermo Scientific™ Pierce™ Recombinant Protein G
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
Brand: Thermo Scientific™ 77676
Description
Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.Highlights:
- Contains two Fc-binding domains per protein
- Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
- Poorer than Protein A for guinea pig, pig, dog and cat
- Does not bind human IgM, IgD or IgA
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Specifications
500 mg | |
Powder | |
Antibodies |
Pierce™ | |
Protein G | |
Upon receipt store at -20°C. Product is shipped at ambient temperature. |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
ZF385005 | Certificate of Analysis | 26/06/2024 | 77676 |
YJ383039 | Certificate of Analysis | 24/10/2023 | 77676 |
YH382528 | Certificate of Analysis | 23/08/2023 | C22322.30 |
SPECS | Certificate of Analysis | 12/10/2022 | C22322.30 |
VC297301 | Certificate of Analysis | 25/03/2020 | C22322.30 |
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For Research Use Only. Not for use in diagnostic procedures.