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Thermo Scientific™ Pierce™ Peroxide Assay Reagent C Product Code.: 11881355

Thermo Scientific™ Pierce™ Peroxide Assay Reagent C

Product Code. 11881355 Shop All Thermo Scientific Products
25 mL, 25mL
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Quantity:
25 mL
Unit Size:
25mL
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Product Code. 11881355

Brand: Thermo Scientific™ 23283

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This item is not returnable. View return policy

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement. The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Highlights:

  • Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples

  • Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself

  • Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes

  • Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples

Recommended for:

General hydrogen peroxide (H2O2) measurement in protein samples; Quantitation of peroxides in detergents; Assessment of the effects of peroxides on cellular activity; Determination of protein glycation; Measurement of peroxide accumulation in lipids

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Description Pierce Peroxide Assay Reagent C
Assay Peroxide Assay Reagent C
Detection Method Colorimetric
Product Line Pierce™
Product Type Quantitative Peroxide Assay
Specificity Not Target-Specific
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 25 mL

Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZG381903 Certificate TypeCertificate of Analysis Date07/08/2024 Product Code23283
Lot NumberZA386452 Certificate TypeCertificate of Analysis Date09/02/2024 Product Code23283
Lot NumberYK383506 Certificate TypeCertificate of Analysis Date25/01/2024 Product Code23283
Lot NumberYG372343 Certificate TypeCertificate of Analysis Date10/08/2023 Product Code23283
Lot NumberYC369117 Certificate TypeCertificate of Analysis Date06/04/2023 Product Code23283

For Research Use Only. Not for use in diagnostic procedures.

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