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Thermo Scientific™ Pierce™ NHS-Activated Agarose, Dry Product Code.: 10150175
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Thermo Scientific™ Pierce™ NHS-Activated Agarose, Dry

Product Code. p-4531483 Shop All Thermo Scientific Products
1 g, 1g
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Quantity:
1 g
5 g
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Product Code. 10150175

Brand: Thermo Scientific™ 26196

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769.37 NOK valid until 2025-07-04
Use promo code "24112" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Description

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Perform rapid and stable immobilization of proteins, peptides and other ligands with Thermo Scientific™ Pierce NHS-Activated Agarose, a high-quality beaded-agarose support.

The activated affinity resin is available as a slurry or dry powder, allowing simple and efficient immobilization of proteins to a beaded-agarose support. The beaded agarose support contains N-hydroxysuccinimide (NHS) functional groups that react with primary amines to form stable amide linkages. The coupling reaction is performed in an amine-free buffer at pH 7 to 9. Protein coupling efficiency is typically greater than 80%, regardless of the molecular weight or pI the ligand. Coupling capacity exceeds 25mg of protein per milliliter of resin. Once a ligand is immobilized, the prepared resin can be used for multiple affinity purification procedures. The crosslinked beaded agarose has fast linear flow potential, making it useful for gravity-flow and low- to medium-pressure applications.

The Pierce NHS-Activated Agarose resin is available in an anhydrous acetone slurry and as dry powder. The unique dry form does not require storage in or removal and disposal of the acetone solvent. In addition, the dry resin is ideal for coupling reactions with dilute samples because it concentrates the sample as the resin swells, reducing the volume of the starting material and resulting in highly efficient ligand immobilization.

Highlights:

  • Easy to use – immobilize in a simple one-step reaction with minimal hands-on time

  • Rapid and efficient – greater than 85% coupling for most proteins within 30 minutes

  • Innovative – the dry agarose concentrates the sample, making it ideal for immobilizing dilute proteins

  • Safe – no hazardous chemicals needed (e.g., sodium cyanoborohydride, cyanogen bromide)

  • Versatile – affinity resin is adaptable to column and batch affinity chromatography techniques and FPLC applications

  • Compatible – use with any primary amine-containing compound

  • Reusable – the leak-resistant chemistry means you can reuse the affinity resin

  • High binding capacity – binding capacity of >30mg/mL (slurry) or >25mg/mL (dry resin)

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) ≥25 mg per mL Coupling
Content And Storage Upon receipt store dry resin at -20°C. Product is shipped in an ice pack.
Product Line Pierce™
Quantity 1 g
Protein Form Recombinant
Target Antibody, Protein, Peptide, Ligand
Type Agarose
Stationary Phase NHS Ester
Format Vial
Column Type Affinity, Agarose Resin

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Product Code
Lot NumberAA402811 Certificate TypeCertificate of Analysis Date31/01/2025 Product Code26196
Lot NumberZI400208 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code26196
Lot NumberSPECS Certificate TypeCertificate of Analysis Date15/10/2024 Product Code26196
Lot NumberZG397949 Certificate TypeCertificate of Analysis Date07/08/2024 Product Code26196
Lot NumberZF395219 Certificate TypeCertificate of Analysis Date12/07/2024 Product Code26196

For Research Use Only. Not for use in diagnostic procedures.

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