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Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL) Product Code.: 11835135
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Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL)

Product Code. 11835135 Shop All Thermo Scientific Products
150 μL, 150µL
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Quantity:
150 μL
Unit Size:
150µL
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Product Code. 11835135

Brand: Thermo Scientific™ 88216

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1037.38 NOK valid until 2025-07-04
Use promo code "24112" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Description

Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.

Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates. Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Highlights:

  • Digests nucleic acids (DNA and RNA)

  • Effective for double-stranded, single-stranded, circular and linear nucleic acids
  • Active in neutral to alkaline buffer conditions containing divalent calcium ions
  • Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol

Compatible with:

Thermo Scientific Subcellular Protein Fractionation Kit (Part No. 78840); Thermo Scientific Pierce ChIP Kit (Part No. 26156)

Recommended for:

Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures

TRUSTED_SUSTAINABILITY

Specifications

Product Type Cell Lysis Enzyme
Content And Storage Upon receipt store at -20°C. Product is shipped with dry ice.
Form Liquid
Reagent Type Enzyme for Cell Lysis
Quantity 150 μL

Certificates

Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZJ403834 Certificate TypeCertificate of Analysis Date16/12/2024 Product Code88216
Lot NumberZJ403834A Certificate TypeCertificate of Analysis Date16/12/2024 Product Code88216
Lot NumberZI397833 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code88216
Lot NumberZI397833A Certificate TypeCertificate of Analysis Date15/10/2024 Product Code88216
Lot NumberZH395915 Certificate TypeCertificate of Analysis Date20/08/2024 Product Code88216

For Research Use Only. Not for use in diagnostic procedures.

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