Thermo Scientific™ Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible

Description

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:

• Chelator stable—stable in buffer containing 20 mM EDTA and DTT
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
• High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.