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Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit
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The Thermo Scientific™ Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.
Highlights:
Sensitive – 1000 times greater sensitivity allows utilization of smaller numbers of cells
Cost effective – highest sensitivity assays result in decreased reagent consumption
Secreted luciferase – allows real-time assays and kinetic studies without destroying cells
Time-saving – assays using secreted luciferases require minimal sample handling
Automation-friendly – amenable to high throughput screens
Convenient – contains a universal cell lysis buffer and optimized flash assay reagent
Safe – allows one to perform non-radioactive assays
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Specifications
Specifications
| Type | Assay Kit |
| Target | Luciferase, Gaussia Luciferase |
| Sufficient For | 100 Luciferase Assays |
| Quantity | 100 Reaction Kit |
| Content And Storage | Sufficient For: 100 luciferase assays in microplate wells • Gaussia Flash Assay Buffer, 5 mL (store at 4°C) • 100X Coelenterazine, 50 μL (store at -80°C) • Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature) Kit may be stored at -80°C, or individually at indicated temperatures. |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Format | 384-well plate, 96-well plate |
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Certificates
Certificates
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Safety and Handling
- Pierce Gaussia Luciferase Flash Assay Kit
- Danger
- Acute toxicity Category 3
- Flammable liquid Category 2
- Specific target organ toxicity after single exposure Category 1
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : methanol
For Research Use Only. Not for use in diagnostic procedures.
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